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Registro Completo |
Biblioteca(s): |
Embrapa Pecuária Sudeste. |
Data corrente: |
28/08/2018 |
Data da última atualização: |
12/09/2018 |
Tipo da produção científica: |
Artigo em Periódico Indexado |
Autoria: |
TOSCANO, J. H. B.; LOPES, L. G.; GIRALDELO, L. A.; SILVA, M. H. da; OKINO, C. H.; CHAGAS, A. C. de S. |
Afiliação: |
João Henrique Barbosa Toscano, UNESP; Louyse Gabrielli Lopes, UNICEP; Luciana Aparecida Giraldelo, UNICEP; Matheus Henrique da Silva, UNICEP; CINTIA HIROMI OKINO, CPPSE; ANA CAROLINA DE SOUZA CHAGAS, CPPSE. |
Título: |
Identification of appropriate reference genes for local immune-related studies in Morada Nova sheep infected with Haemonchus contortus. |
Ano de publicação: |
2018 |
Fonte/Imprenta: |
Molecular Biology Reports, v.45, n.5, p.1253-1262, out. 2018. |
Idioma: |
Inglês |
Conteúdo: |
Due to the great economic impact of Haemonchus contortus on sheep farming, there is an increasing number of studies addressing host resistance against this nematode, including identification of directly related immune mechanisms. In this context, relative gene expression by RT-qPCR have been largely used, due to its rapidity, high sensitivity, specificity, and reproducibility. Although, appropriate reference gene selection is crucial for accurate interpretation of results. In this study, five reference genes (GAPDH, G6PDH, YWHAZ, ACTB, and B2M) were tested for expression stability in abomasum (fundic and pyloric regions) and abomasal lymph nodes of Morada Nova sheep classified as resistant (n = 5) or susceptible (n = 5) to H. contortus infection in a flock of 151 animals. GAPDH combined with YWHAZ were selected as reference genes for abomasal fundic region and abomasal lymph nodes, whereas YWHAZ was the most stable gene for abomasal pyloric region. These genes presented the lowest intra- and inter-group variations and, consequently, highest stability. In contrast, expression of G6PDH was the least stable in all tissues. The impact of reference gene selection was demonstrated by relative quantification of a pro-inflammatory cytokine (TNFα) in abomasal fundic region. Significant differences in TNFα expression levels between resistant and susceptible groups were only observed when the most stable genes (GAPDH combined with YWHAZ) or GAPDH were used as reference genes, whereas no significant differences were observed when other tested reference genes were used. It was demonstrated that normalization of expression data using inappropriate reference genes may significantly influence interpretation results. MenosDue to the great economic impact of Haemonchus contortus on sheep farming, there is an increasing number of studies addressing host resistance against this nematode, including identification of directly related immune mechanisms. In this context, relative gene expression by RT-qPCR have been largely used, due to its rapidity, high sensitivity, specificity, and reproducibility. Although, appropriate reference gene selection is crucial for accurate interpretation of results. In this study, five reference genes (GAPDH, G6PDH, YWHAZ, ACTB, and B2M) were tested for expression stability in abomasum (fundic and pyloric regions) and abomasal lymph nodes of Morada Nova sheep classified as resistant (n = 5) or susceptible (n = 5) to H. contortus infection in a flock of 151 animals. GAPDH combined with YWHAZ were selected as reference genes for abomasal fundic region and abomasal lymph nodes, whereas YWHAZ was the most stable gene for abomasal pyloric region. These genes presented the lowest intra- and inter-group variations and, consequently, highest stability. In contrast, expression of G6PDH was the least stable in all tissues. The impact of reference gene selection was demonstrated by relative quantification of a pro-inflammatory cytokine (TNFα) in abomasal fundic region. Significant differences in TNFα expression levels between resistant and susceptible groups were only observed when the most stable genes (GAPDH combined with YWHAZ) or GAPDH were used as reference genes,... Mostrar Tudo |
Palavras-Chave: |
Genes de referência. |
Thesagro: |
Haemonchus Contortus; Ovino. |
Thesaurus Nal: |
Sheep. |
Categoria do assunto: |
G Melhoramento Genético |
Marc: |
LEADER 02463naa a2200229 a 4500 001 2094611 005 2018-09-12 008 2018 bl uuuu u00u1 u #d 100 1 $aTOSCANO, J. H. B. 245 $aIdentification of appropriate reference genes for local immune-related studies in Morada Nova sheep infected with Haemonchus contortus.$h[electronic resource] 260 $c2018 520 $aDue to the great economic impact of Haemonchus contortus on sheep farming, there is an increasing number of studies addressing host resistance against this nematode, including identification of directly related immune mechanisms. In this context, relative gene expression by RT-qPCR have been largely used, due to its rapidity, high sensitivity, specificity, and reproducibility. Although, appropriate reference gene selection is crucial for accurate interpretation of results. In this study, five reference genes (GAPDH, G6PDH, YWHAZ, ACTB, and B2M) were tested for expression stability in abomasum (fundic and pyloric regions) and abomasal lymph nodes of Morada Nova sheep classified as resistant (n = 5) or susceptible (n = 5) to H. contortus infection in a flock of 151 animals. GAPDH combined with YWHAZ were selected as reference genes for abomasal fundic region and abomasal lymph nodes, whereas YWHAZ was the most stable gene for abomasal pyloric region. These genes presented the lowest intra- and inter-group variations and, consequently, highest stability. In contrast, expression of G6PDH was the least stable in all tissues. The impact of reference gene selection was demonstrated by relative quantification of a pro-inflammatory cytokine (TNFα) in abomasal fundic region. Significant differences in TNFα expression levels between resistant and susceptible groups were only observed when the most stable genes (GAPDH combined with YWHAZ) or GAPDH were used as reference genes, whereas no significant differences were observed when other tested reference genes were used. It was demonstrated that normalization of expression data using inappropriate reference genes may significantly influence interpretation results. 650 $aSheep 650 $aHaemonchus Contortus 650 $aOvino 653 $aGenes de referência 700 1 $aLOPES, L. G. 700 1 $aGIRALDELO, L. A. 700 1 $aSILVA, M. H. da 700 1 $aOKINO, C. H. 700 1 $aCHAGAS, A. C. de S. 773 $tMolecular Biology Reports$gv.45, n.5, p.1253-1262, out. 2018.
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Embrapa Pecuária Sudeste (CPPSE) |
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Registro Completo
Biblioteca(s): |
Embrapa Milho e Sorgo. |
Data corrente: |
28/10/2010 |
Data da última atualização: |
18/07/2018 |
Tipo da produção científica: |
Artigo em Anais de Congresso |
Autoria: |
ROCHA, K. R.; ANDRADE-NOBREGA, G. M.; FIGUEIREDO, J. E. F.; MARRIEL, I. E.; MEIRELLES, W. F.; PACCOLLA-MEIRELLES, L. D. |
Afiliação: |
KATIA R. ROCHA, UEL; GISELE M. ANDRADE-NOBREGA, UEL; JOSE EDSON FONTES FIGUEIREDO, CNPMS; IVANILDO EVODIO MARRIEL, CNPMS; WALTER FERNANDES MEIRELLES, CNPMS; LUZIA DORETTO PACCOLA-MERELLES, UEL. |
Título: |
Caracterização comparativa entre isolados de Pantoea ananatis oriundos de lesões de capim-colchão com aqueles de lesões da Mancha Branca do Milho. |
Ano de publicação: |
2010 |
Fonte/Imprenta: |
In: CONGRESSO NACIONAL DE MILHO E SORGO, 28.; SIMPÓSIO BRASILEIRO SOBRE A LAGARTA DO CARTUCHO, 4., 2010, Goiânia. Potencialidades, desafios e sustentabilidade: resumos expandidos... Sete Lagoas: ABMS, 2010. |
Descrição Física: |
1 CD-ROM. |
Idioma: |
Português |
Palavras-Chave: |
Nucleação do gelo; RAPD; Seqüenciamento. |
Thesagro: |
Zea Mays. |
Categoria do assunto: |
-- |
URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/item/25066/1/0151.pdf
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Marc: |
LEADER 00888nam a2200217 a 4500 001 1865518 005 2018-07-18 008 2010 bl uuuu u00u1 u #d 100 1 $aROCHA, K. R. 245 $aCaracterização comparativa entre isolados de Pantoea ananatis oriundos de lesões de capim-colchão com aqueles de lesões da Mancha Branca do Milho.$h[electronic resource] 260 $aIn: CONGRESSO NACIONAL DE MILHO E SORGO, 28.; SIMPÓSIO BRASILEIRO SOBRE A LAGARTA DO CARTUCHO, 4., 2010, Goiânia. Potencialidades, desafios e sustentabilidade: resumos expandidos... Sete Lagoas: ABMS$c2010 300 $c1 CD-ROM. 650 $aZea Mays 653 $aNucleação do gelo 653 $aRAPD 653 $aSeqüenciamento 700 1 $aANDRADE-NOBREGA, G. M. 700 1 $aFIGUEIREDO, J. E. F. 700 1 $aMARRIEL, I. E. 700 1 $aMEIRELLES, W. F. 700 1 $aPACCOLLA-MEIRELLES, L. D.
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